Recombinant PD-L1 anticorps

N° du produit ABIN7543101

L’anticorps Lapin Chimeric anti-PD-L1 a été validé pour FACS, IHC, ELISA et BP. Il convient pour détecter PD-L1 dans des échantillons de Souris.

Aperçu rapide pour Recombinant PD-L1 anticorps (ABIN7543101)

Antigène: PD-L1 (CD274 (PD-L1))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp PD-L1 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Blocking Peptide (BP)

Clone: 10F-9G2

Détail du produit anti-PD-L1 anticorps

Fonction: Anti-PD-L1 [10F.9G2], Rabbit IgG, kappa

Specificité: This antibody is specific for murine PD-L1.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD-L1 CHO transfectants.

Isotype: IgG kappa

Information d'application

Indications d'application: The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur PD-L1

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: PD-L1

Sujet: CD274, PDL1, PD L1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, 10F9G2, 10F 9G2

UniProt: Q9EP73

Pathways: Cancer Immune Checkpoints